To better test the effects on locomotor activity in response to certain compounds, zebrafish larvae can be loaded into multi-well plates. For example, Duan et al. (2013) investigated the developmental toxicity of quantum dots in larvae using 24-well plates in a DanioVision system.
Quantum dots are nanoparticles made of a semiconductor material, that have unique optical properties: the wavelength (and thus color) of their light emission depends on their size rather than the material they are made of. As a result, they are of great interest for fields such as biological imaging, medical diagnostics, drug delivery, and gene therapy.
Certain quantum dots may be toxic, limiting their usefulness. Duan investigated a specific type of quantum dots called Cadmium Telluride (CdTe) in a zebrafish study.
The researchers looked at activity levels of 6 dpf larvae swimming in visible light in a DanioVision Observation Chamber. Using EthoVision XT, they found that the total swimming distance was suppressed in treated larvae in a dose-dependent manner.
In a light-dark experiment (10-minute bouts of alternating light conditions), the control group showed the typical increased activity levels during dark phases. In comparison, the treated larvae showed significant hypo-activity.
Read more about this study on our blog.
Duan, J.; Yu, Y.; Li, Y.; Yu, Y.; Li, Y.; Huang, P.; Zhou, X.; Peng, S.; Sun, Z. (2013). Developmental toxicity of CdTe QDs in zebrafish embryos and larvae. Journal of Nanoparticle Research, 15, 1700.