How do I use EntoLab?

EntoLab is an integrated hardware/software system consisting of a compact observation chamber and a Windows software suite.

The EntoLab software suite consists of a range of software programs for video tracking, camera settings optimization, video streaming, and data processing and analysis.

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EntoLab test array

Developed in collaboration with

Wageningen University and Research Royal van Zanten Nunhems Syngenta East-West Seed Advanta Enza Zaden Limagrain Europe

Which hardware do I need?

The EntoLab setup is a convenient benchtop system that will enrich your screening facility. The equipment can also be mounted on a cart with integrated computer equipment, providing a mobile setup that can easily move between labs. The EntoLab system includes:

  • checkCompact benchtop cabinet
  • checkHigh-resolution, light-sensitive color video camera (2040x2046 pixels) with 1" 25mm lens
  • checkVertically adjustable camera mount
  • checkLED arrays for overhead illumination and backlight panel
  • checkRemovable Fresnel lens to rectify viewing angle
  • checkAnti-fogging cover plate and lateral ventilation unit, with adjustable power supply
EntoLab hardware setup

EntoLab observation chamber

With EntoLab, you can standardize your test environment, including multi-arena assay plates and illumination, and collect your data efficiently. The EntoLab Observation Chamber is designed in such a way to maximize image quality, reliability of tracking and throughput. Furthermore, the EntoLab hardware is easy to use and clean.

EntoLab observation chamber

Assay plates for insect research

Assay plates

Assay plates

EntoLab offers a modular system of assay plates that can track a variety of insects, plants, and substrates, with up to 300 arenas on a single plate. A key feature is the synchronized release of insects from a cage plate beneath the arena plate, ensuring a uniform start for all trials.

Foam assay plates

Foam assay plates

The foam assay plates consist of a foam sample support plate and a top arena plate. The support plate cushions test samples like leaf fragments and has 126 holes for insects to emerge into the arena. Depending on the arena plate shape, insects encounter a sample on one side (no-choice) or both sides (choice).

Plexiglass assay plates

Plexiglass assay plates

Plexiglass assay plates are designed for thicker samples like fruits, stems, or agar. They feature a support plate with chambers for the samples and 126 holes for insects to emerge. Insects will find either a single sample (no-choice) or two different samples (2-choice) after being released.

What does the software offer me?

EthoVision XT screenshot EntoLab

EthoVision XT

The EntoLab system comes with EthoVision XT, the renowned computer vision and video tracking software. EthoVision XT processes each high-resolution image in the digital video file, detects the insect against the background in each arena, extracts its body center, and generates a track file for each arena.

Due to its superior object detection algorithms, EthoVision XT can handle the most challenging insect-plant combinations. EthoVision XT can process a single video file or a batch of files originating from multiple EntoLab chambers, in a fully automated fashion.

EthoAnalysis screenshot EntoLab

EthoAnalysis

Track files are imported into EthoAnalysis, a specialized software package developed for the analysis of insect behavior recorded with EthoVision XT. Based on various user settings, which can be specific for an insect species, EthoAnalysis translates the raw track data into series of zone-specific behaviors of three categories: halting, moving, and not-detected.

From these time series, various behavior statistics are computed, either for the complete trial or per zone, per hour, or per behavior category, including duration (short/medium/long) or movement velocity (slow/medium/fast).

Interested in EntoLab?

Get in touch!

Want to learn more about setting up EntoLab for your insect behavior research? Contact us for a demo or pricing information.

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