Knowledge Base

Manual Classification

1. Under Trial & Run Selection, select a trial and one of the runs in that trial, or click the Previous/Next button to navigate to the trial and run you want to classify. The associated video opens with the video frame that contains the first unlabeled print.
2. Do one of the following:
   • Continue at the next step to start assigning labels.
   • Move the video to a new position by using the playback buttons (see page 87 for how to move through the video).
   • Optionally, zoom in to get a better view of the prints (see page 84 for how to zoom in and out in the Video window).
3. To label a print, do the following:
   1. Using the mouse, select a rectangle or draw a larger box around one of the white rectangles and release the mouse button.
   2. Select a label from the menu.
   3. Assign a label to other unlabeled prints in this frame until there are no unlabeled prints left or you have labeled all relevant prints in this frame.

   The labeled print appears under Results, both in the Print View and Timing View of the Graphical tab and in the Numerical tab.

   The following labels can be used:

   • Paws: RF = Right Front, RH = Right Hind, LF = Left Front, LH = Left Hind
   • Hip and Knee: RM = Right Hip, RK = Right Knee, LM = Left Hip, LK = Left Knee
   • Other body parts: NO = Nose, AB = Abdomen, TA = Tail, GT = Genitalia
4. The video now automatically moves to the next frame with unlabeled prints that do not overlap with already labeled prints. You can now do one of the following:
   • Repeat the procedure described in step 3 above.
   • Click the Jump to Next Unlabeled Print button or the Jump to Previous Unlabeled Print button to go to a next or previous specific unlabeled print and repeat the procedure described in step 3 above.

   You can speed up labeling prints by drawing a box around both labeled prints and unlabeled prints that belong to the same paw. The unlabeled prints are automatically assigned the same label.

5. Continue until you have labeled all relevant prints in the run.

If you labeled the complete run, the Classification Finished Tasks window appears. To continue, select one of the options in this window.

Zoom In/Out in the Video Window

During classification, you can zoom in in the Video window to get a better view of the paw prints. The available zoom factors are: 1x, 2x, 3x, 4x, and 5x.

To zoom, do one of the following:

• Click the Zoom In button or the Zoom Out button.
• To zoom in, press Ctrl+'+' (numeric keyboard); to zoom out, press Ctrl+'-' (numeric keyboard).
• To jump to the normal view, press Ctrl+'0' (zero on the numeric keyboard).

Combine Stances

Occasionally, a print in a frame may not be detected. As a result, the stance (that is, contact of a paw with the glass plate during one paw placement) of a specific paw is subdivided into two separate stances. These separated stances can be manually combined.

If you have a lot of subdivided stances, it might be that your Green Intensity Threshold was set too high. Try decreasing the Green Intensity Threshold before proceeding with combining stances.

Procedure

To combine two stances, right-click on the first stance and select Combine with Next. The stances are combined and the new stance is considered to be a single stance in both the Visualization and the Run Statistics.

CatWalk is not able to calculate the Stand Index for the new stance when the Max Contact point is in the first stance.

The menu also provides an option to exclude a stance from analysis. This option is used to mark the stance as junk.

Label Junk

When a print is displayed that you are sure is not part of the animal's body (for example, a dropping or dirt from the animal's body) or is irrelevant, you can label it as Junk. You do this in exactly the same way as assigning one of the other labels. Prints labeled as Junk are not displayed in the Print View and Timing View.

If you have a lot of noise prints, it might be better to first increase the Green Intensity Threshold.

I Have a Lot of Prints That Are Junk

In cases where a frame contains a lot of junk, you can do the following to speed up labeling:

1. Label all relevant prints in the frame.
2. Click the UN = Junk button (alternatively, press the '~' key or the '-' (en dash) key on your keyboard) to label all remaining prints in the frame as Junk.

I Want to Permanently Remove Prints Labeled as Junk

When you have labeled prints as Junk, they still appear in the Video window when you replay a run. If you do not want Junk prints to be displayed, you can permanently remove them:

1. Carry out the two steps described under I Have a Lot of Prints That Are Junk above.
2. Click the Remove All Junk Prints button.

Removing all Junk prints cannot be undone. The only way to restore prints is to manually add a print in each frame from which a Junk print was removed.

Add Paw Prints

You may want to add a print when there is no rectangle around a print whose intensity exceeds the Green Intensity Threshold. This can occur in the following situations:

• You have manually removed a print labeled as Junk.
• In the Acquisition Settings, you selected the option Discard Incomplete Prints at the Start and End of a Run, which results in no rectangle being drawn around the print.

Procedure

1. Draw a box around the print.
2. From the menu, select Add Print.