Knowledge Base

14 Analyze Data

Analysis Profiles And Options

Analysis Profiles in the template experiment

Distance, Time & Movement - Contains the dependent variables

Distance moved, Velocity and Movement.

Path Shape - Contains the dependent variables Heading, Turn angle,

Angular velocity and Meander.

Rotation - Contains two instances of the dependent variable

Rotation, to count the number of clockwise and counterclockwise rotations. For more information on these and other dependent variables, see

Dependent Variables in Detail in the EthoVision XT Help. Also see

Analysis of Trial Control data in the Reference Manual - Trial and

Hardware Control in EthoVision XT.

Analyze intervals

Make sure you have defined a Data profile which specifies the intervals you are interested in (see page 106). Next, in the Analysis profile choose your variables (e.g. Distance moved). Then visualize the data (see an example in Figure 15) and run analysis (see page 115).

Activity analysis

You can also use the dependent variable Activity state. This variable is

only available when you selected Activity analysis in the Experiment

Settings (see page 46 and page 66). With Activity state you can calculate how long and how frequent your subject has been in different activity states. These states depend on the total pixel change within the arena between a sample and the previous sample. It is important that all defined arenas have exactly the same size. The number of states (two to four) and their thresholds are user- defined.

Analyze convulsions

To analyze convulsions, you can use several variables, such as Velocity, Activity and Mobility. If you use Mobility, start with setting a High Mobility Threshold to a high value like 95%, and an averaging interval of 2. Compare the scores of Highly Mobile with those of convulsions obtained manually. Figure 15 Example of visualization of data after nesting over a Trial Control State. Here the samples of Arena 1 are displayed in full color when the white light was on. Only those samples are subject to analysis. The first plot is of the variable Trial Control State (defined as: From Light on to Light off; it illustrates the effect of nesting), and the second is of Distance moved defined in the Analysis profile.

Calculate The Statistics

The instructions below describe the general procedure of selecting a dependent variable and calculating statistics for that variable. 1. Choose Analysis > Analysis Profile. In the Analysis Profiles window, select New and type a name for the new profile, then click OK. 2. Click the Add button next to the dependent variable you want to use

for analysis (for an overview, see Dependent Variables in Detail in

the EthoVision XT Help). 3. In the window that appears, select the properties of the dependent variable.

In the Trial Statistics tab, choose the statistics you want to calculate

per trial for that variable. If you run multiple trials, select additional statistics in the Group Statistics tab. 4. Repeat steps 2-3 to add more dependent variables. 5. Choose Analysis > Results > Statistics & Charts. Choose the Data Profile from the list on the tool bar and click the Calculate button. 6. The results appears on the screen. The Trial Statistics tab shows the

statistics per trial. The Group Statistics & Charts tab shows the

statistics and charts from the summarized results of all trials. See

Calculate Statistics in the EthoVision XT Help for how to read and

customize your results. 7. optional Click the Layout button to modify the layout of the results table.

Batch calculations

It is also possible to carry out multiple analyses with different filters, data profiles and analysis profiles. To do so click the Batch button.

Make your selection from the lists under Select the profile

combinations to calculate and click Add. When done, click Calculate. To

view a specific analysis result, select the options from the lists on the

toolbar. For more information on batch statistics calculation, see Batch

statistics calculation in the EthoVision XT Help.

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Source: Reference Manual - DanioVision DVOC-0041