Knowledge Base

2 What's New In DanioVision

Daniovision Observation Chamber

For users of DanioVision DVOC-0040

The white light LED array has been improved. The White light can be set to higher intensity.

For users of DanioVision DVOC-0030

The housing has been completely redesigned. You open the observation chamber by tilting the lid along the top side. This way you have easier access to the camera and opening/closing the lid produces less vibration.

note The DanioVision Observation Chamber is designed to block

light from the outside. However, it cannot be guaranteed that it is completely dark inside the Chamber. For experiments that require absolute darkness (like experiments with day-night rhythms), place the DVOC in a dark room. The housing is now larger to create more space for accessing the video camera or adding devices. For this purpose, the housing can be disassembled more easily than before. For the instructions see page 116. The basin has been redesigned. It has now a more flexible retainer that can hold a wider range of well plates. DanioVision can now work with ANSI, SBS compatible micro plates (L =127.76± 0.5 mm, W=85.48 ± 0.5 mm) with a maximum height of 27 mm, and with Petri dishes with a diameter up to 90 mm. To place small well plates and petri dishes, slide the bridge to a new position in such a way that the well plate/petri dish is in the middle of the camera

view (left). For details see place well plates and petri dishes on

page 37. The mechanism for moving the Fresnel lens located above the well plate has been improved. You can now move the lens all the way out of the camera view when you need to place containers higher than 27 mm. Communication between DanioVision and EthoVision XT has improved via a new version of the hardware interface software. See install ethovision xt on page 22.

All four TTL lines TTL 1 to TTL 4 are available for communication with

external devices. For connecting custom hardware, additional

expansion connectors Expansion 1 and 2 are available. See Figure 3

on page 15. Figure 2 Positioning a well plate (left) and a petri dish (right).

The White Light is now dimmable at very low intensity, without the

need to operate the Low light switch located behind the camera.

The DanioVision Tapping Device has been designed to provide

DanioVision with a reliable device which evokes a startle response in zebrafish larvae. See The DanioVision Tapping Device on page 72.

note The DanioVision Tapping Device is a separate add-on to the

DanioVision system.

For users of DanioVision DVOC-002x

The DanioVision camera is now a Gigabit Ethernet (GigE) digital camera connected to the EthoVision XT computer through a 1-Gb Ethernet card. With a GigE camera, tracking can be done with a higher sample rate and higher resolution. The intensity of the infrared LED back-lighting has been increased. This results in a more even distribution of back-lighting over the well-plate and a higher contrast between animals and the background. Therefore, it is easier to configure the detection settings and detection is better. It is possible to use very low light levels for the DanioVision White Light in addition to the default, standard White Light levels. The standard White light levels range from ±20 to ±10000 lux. The low White light levels range from 0 to ±20 lux. The anti-condensation mechanism can now be easily switched on and off, or set to automatic. The anti-condensation mechanism prevents the lens from condensing during an experiment.

Ethovision Xt

Here the main additions relevant to DanioVision are listed. For a complete list of the new features, see the EthoVision XT Help "What's new" section.

For users of EthoVision XT 15

Improved Subject Contour filter. In the Detection Settings, a second

Erosion filter is added to the Subject Contour settings. This filter is

added after the pre-existing Erosion and Dilation, and improves detection in some difficult situations. For more information, see

Advanced detection settings: Subject contour in the EthoVision XT

Help. Trial Control variable in the analysis. In the Analysis profile you can now select the Trial Control variables used in the Trial Control protocols. This way you can analyze or visualize its values just like the other dependent variables. You can also visualize a Trial Control variable in the Integrated Visualization for testing purposes. For example, to check that a variable gets the expected value at a specific time in the trial.

note The Deep learning functionality in EthoVision XT 16 is not

designed specifically to track fish larvae. In DanioVision experiments, always choose Contour-based as the body point detection technique.

For users of EthoVision XT 14

Heatmaps are plotted together with a time scale. This helps you quantify the time that the subjects spend in specific locations.

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Source: Reference Manual - DanioVision DVOC-0041