Knowledge Base

Analysis Profiles and Options

Analysis Profiles in the Template Experiment

The following analysis profiles are available in the template experiment:

• Distance, Time & Movement - Contains the dependent variables Distance moved, Velocity and Movement.
• Path Shape - Contains the dependent variables Heading, Turn angle, Angular velocity and Meander.
• Rotation - Contains two instances of the dependent variable Rotation, to count the number of clockwise and counterclockwise rotations.

For more information on these and other dependent variables, see Dependent Variables in Detail in the EthoVision XT Help. Also see Analysis of Trial Control data in the Reference Manual - Trial and Hardware Control in EthoVision XT.

Analyze Intervals

Make sure you have defined a Data profile which specifies the intervals you are interested in. Next, in the Analysis profile choose your variables (e.g. Distance moved). Then visualize the data and run analysis.

Activity Analysis

You can also use the dependent variable Activity state. This variable is only available when you selected Activity analysis in the Experiment Settings.

With Activity state you can calculate how long and how frequent your subject has been in different activity states. These states depend on the total pixel change within the arena between a sample and the previous sample. It is important that all defined arenas have exactly the same size. The number of states (two to four) and their thresholds are user-defined.

Analyze Convulsions

To analyze convulsions, you can use several variables, such as Velocity, Activity and Mobility. If you use Mobility, start with setting a High Mobility Threshold to a high value like 95%, and an averaging interval of 2. Compare the scores of Highly Mobile with those of convulsions obtained manually.

Calculate the Statistics

The instructions below describe the general procedure of selecting a dependent variable and calculating statistics for that variable.

1. Choose Analysis > Analysis Profile. In the Analysis Profiles window, select New and type a name for the new profile, then click OK.
2. Click the Add button next to the dependent variable you want to use for analysis (for an overview, see Dependent Variables in Detail in the EthoVision XT Help).
3. In the window that appears, select the properties of the dependent variable. In the Trial Statistics tab, choose the statistics you want to calculate per trial for that variable. If you run multiple trials, select additional statistics in the Group Statistics tab.
4. Repeat steps 2-3 to add more dependent variables.
5. Choose Analysis > Results > Statistics & Charts. Choose the Data Profile from the list on the tool bar and click the Calculate button.
6. The results appear on the screen. The Trial Statistics tab shows the statistics per trial. The Group Statistics & Charts tab shows the statistics and charts from the summarized results of all trials. See Calculate Statistics in the EthoVision XT Help for how to read and customize your results.
7. Optional: Click the Layout button to modify the layout of the results table.

Batch Calculations

It is also possible to carry out multiple analyses with different filters, data profiles and analysis profiles. To do so click the Batch button. Make your selection from the lists under Select the profile combinations to calculate and click Add. When done, click Calculate. To view a specific analysis result, select the options from the lists on the toolbar. For more information on batch statistics calculation, see Batch statistics calculation in the EthoVision XT Help.

Open the DanioVision Casing

Tools You Need

A Phillips or (preferably) a Pozidrive screwdriver, size 2. Do not use electric screwdrivers.

Bench Plate

Remove the bench plate, for example, to install the Tapping Device and other custom hardware you want to place inside the DanioVision Observation Chamber. Also when opening the top casing it is handy to first remove the bench plate.

1. Remove the four screws as indicated in the picture below.
2. Remove the bench plate.

Top Casing

Remove the top casing, for example, when you need to replace the camera.

1. Optional: Remove the bench plate first, as this makes the following steps easier.
2. Remove the eight screws as indicated in the picture below. Note that the screws located more internally are shorter than the others. These are attached to the aluminium frame.
3. Close the lid and lift the top casing.
4. When putting the top casing back in place, make sure that you insert the sides into the bottom casing as indicated in the picture below. Then, tighten all the screws.

Sides and Back Casing

Remove the sides and back casing to access the connector box, wirings and internal tubings. In most cases you do not need to do so.

1. Follow the instructions to remove the top casing.
2. Put the DanioVision Observation Chamber just over the edge of the table and remove the three screws from the bottom plate. Repeat this step for the other side.
3. Remove the two screws at the back as indicated in the picture below.
4. You can now lift the casing.

Connector Panel

To remove the connector panel at the back of the observation chamber, remove the four screws as indicated in the picture below.

Adjusting Lid Friction

You can adjust the friction of the Observation chamber lid. If the lid falls down easily or opens with much friction, it can be adjusted by loosening or tightening the three screws per hinge.

Source: DanioVision DVOC-0041 Reference Manual, Noldus Information Technology