Knowledge Base

Check the DanioVision System (Continued)

Adjusting the Bridge for Well Plates

1. Slide the bridge by one or more positions. Check the video image in EthoVision XT, and make sure that the well plate is centered in the camera view.
2. Fix the screws in the corresponding holes.

Petri Dishes

1. Put the Petri dish against the 90º angle of the bridge.
2. If necessary, remove the screws of the bridge and slide the bridge a few positions until the dish is centered in the camera view.

With DanioVision you can use Petri dishes of diameter up to 90 mm.

We recommend not letting water flow in the basin when using Petri dishes. Water may flow underneath the bottom of the dish, making it shift from its original position during a trial.

Control Evaporation

Wells on the sides of the well plate tend to show a higher rate of evaporation than wells in the center. Also, wells at the front have a higher evaporation rate than those at the back.

Our Recommendations

In long experiments (greater than 24 hours), evaporation may occur for both the wells in the plate and the basin.

• Wells: Check the wells every 24 hours and, if necessary, refill them.
• Basin: Refill the basin every 2–3 days using the Basin Refill tool. For details, see the Reference Manual - Temperature Control Unit DV-TCU.

The environmental conditions (temperature, relative humidity, and air flow) and experimental conditions (lens heater on/off, white light intensity) influence the rate of evaporation and condensation of water from the basin and well plate. If you plan to carry out long experiments, always test evaporation at your environmental and experimental conditions, and plan refills to make sure that the water level never reaches a critical value.

Tip: You can use a special syringe to refill the basin without having to open the DanioVision Observation Chamber. Contact Noldus for more information.

Source: DanioVision DVOC-0041 Reference Manual, Noldus Information Technology