Knowledge Base

Set Up DanioVision (Continued)

Under Configuration Sets, look for the item Default Startup Set. This is the User Set that contains the current settings. Always check that the settings in Pylon Viewer for a particular User Set correspond to those in the table.

Note: EthoVision XT reads the settings from the camera driver software. For a specific EthoVision XT experiment you can override the values of video resolution and frame rate values that are stored in the driver, not other settings. The maximum frame rates have been measured with a 96 well plate setup. Other values may be optimal in other setups.

User Set 1 is the default User Set in most applications. User Set 2 is pre-selected if you purchased a 12-mm lens. User Set 3 is pre-selected if you purchased an 8-mm lens.

For User Set 2, Binning mode must be Summing. For other User Sets either option has no effect with binning equal to 1.

Overview of Predefined User Sets

The following table summarizes the predefined User Sets in the camera driver software and their associated video frame rate, video size, and required camera lens.

For all User Sets: Enable Acquisition Frame Rate must be selected.

Adjust the Camera Settings in EthoVision XT

In EthoVision XT, choose Setup > Experiment Settings and click the camera button. Adjust the settings according to the table above.

For details, see Adjust Camera Settings in EthoVision XT in the EthoVision XT Help.

Adjust the Camera Settings in Pylon Viewer

You can also adjust the camera settings directly in the camera software Pylon Viewer. Adjust the settings according to the configuration you want to use. First, choose a video resolution and frame rate combination, then mount the recommended camera lens, and next make the appropriate settings in Pylon Viewer. Finally, adjust the appropriate settings in EthoVision XT.

For more information about Pylon Viewer, see Configure the Digital Camera in the EthoVision XT Help.

Adjust the following settings in Pylon Viewer:

• Pixel Format (column B in Table 1)
• Analog Gain (column C in Table 1)
• Center X and Center Y options (column D in Table 1)
• Horizontal and Vertical Binning (column E in Table 1)
• Acquisition Frame Rate and Exposure Time (column F in Table 1)

Tip: To preview the camera image, click the Continuous Shot button on the toolbar.

Tip: You can increase the Analog Gain if you need a brighter camera image. However, the image noise will also increase, which may influence tracking.

To save the settings, click the Stop button and open the Configuration Sets item. From the Configuration Set Selector list, select the User Set you want to save the settings to. Next to User Set Save, click Execute. Select the User Set you want to use on a daily basis. From the Default Startup Set list, select the User Set of your choice.

Camera View

Check that the camera view is correct by placing a well plate in the DanioVision Observation Chamber.

Make sure that:

• The well plate is in the middle of the field of view, not rotated or skewed.
• The well plate is in focus. If not, operate the focus ring of the camera lens.
• Fixation screws of all rings on the lens are tightened.
• The aperture is chosen such that the image appears slightly overexposed. With water in the well plate, the image is fine.

When you create an EthoVision XT experiment, check that the Video Resolution, Video Frame Rate, and Color Space match the values in Table 1 for the User Set you want to use.

Source: DanioVision DVOC-0041 Reference Manual, Noldus Information Technology