Knowledge Base

Temperature Control Unit Basin Refill

The Basin Refill Tool

During long-lasting DanioVision experiments, evaporation of water out of the basin could be an issue. You can compensate for evaporation by refilling the basin without opening the DanioVision Observation Chamber (DVOC-0040).

The Basin Refill Tool (DVBR-0010) allows you to refill the basin without opening the DanioVision Chamber during an experiment. It is provided standard with the DanioVision Temperature Control Unit (DVTCU).

It is possible to fill/refill the basin directly (see Chapters 3 and 4 in the Temperature Control Unit – DanioVision User Manual). However, this is not practical when an experiment is running.

Only use the Basin Refill Tool in combination with the DanioVision Temperature Control Unit (DVTCU).

Setting Up the Basin Refill Tool

You must install the Basin Refill Tool before starting the experiment.

1. Make sure your DanioVision basin is empty. To do so, follow the instructions in the section Draining the DanioVision System in Chapter 4 of the Temperature Control Unit – DanioVision User Manual.
2. Disconnect the tube marked in green between the DanioVision Chamber and the TCU (when applicable).
3. Connect the ends of the tubes of the Basin Refill Tool to the corresponding green-marked inlets at the back of the DanioVision Observation Chamber and the Temperature Control Unit.
4. Make sure the faucet is closed (Off position).
5. Fill the syringe with basin liquid, and connect it to the short faucet tube.
6. Fill the basin circuit (see Chapter 3 of the Temperature Control Unit – DanioVision User Manual).

Tip: While the basin circuit is being filled, insert a few ml of liquid from the syringe in order to prevent air bubbles from getting into the circuit.

7. Start the experiment as usual.

How to Use the Basin Refill

How much liquid to insert via the syringe depends on the level of evaporation and the duration of the experiment. Add liquid into the basin circuit whenever needed.

During refill, do not switch off the Temperature Control Unit.

1. Open the faucet (On position).
2. Make sure you can see the overflow outlet of the DanioVision Chamber (red marked tube).
3. Gently push the syringe to insert liquid.
4. Carefully watch the overflow tube. Whenever the liquid comes out, stop pushing the syringe.

Notes:

• Overflow is a good indication of a 100% filled basin; however, it may lag on filling.
• The DVTCU will compensate for any temperature fluctuation due to the refill. However, make sure that the liquid temperature in the syringe is not too far from the desired basin temperature.

To refill the syringe:

1. Make sure the faucet is closed (Off position).
2. Disconnect the syringe.
3. Fill the syringe with basin liquid.
4. Make sure no air is present in the syringe.
5. Connect the syringe back to the faucet tube.

For More Information

For more extensive information on the DanioVision Temperature Control Unit, see the Temperature Control Unit – DanioVision User Manual, which you can find on our website at www.noldus.com/downloads.

Source: DanioVision Temperature Control Unit in a Nutshell, Noldus Information Technology