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EthoVision XT 18 - Application Manual - Version 18

Last updated: Jul 30, 2026

General Information

Setting up the experiment in EthoVision XT

  • Create the experiment
  • Experiment settings
  • Arena settings
  • Trial control settings
  • Detection settings
  • Trial list
  • Protocols on the web featuring EthoVision XT

Acquire the data

  • Protocol
  • Acquire the tracks

Analyze the data

  • Data preparation
  • Visualize the data
  • Calculate the statistics
  • Export the results

The Open Field Test

Introduction

  • The open field for rodents
  • The open field for zebrafish

Physical Setup

The Open Field Experiment in EthoVision XT

  • Experiment settings
  • Manual scoring settings
  • Arena settings
  • Trial control settings
  • Detection settings

Acquiring Data

  • Protocol
  • Score behaviors manually

Data Analysis

  • Analysis profiles
  • Analysis per zone
  • Time variation of behavior
  • Zone crossings
  • Freezing behavior
  • Stretch-attend
  • Rat or mouse behavior recognition
  • Comparing groups
  • Visualizing data

The Morris Water Maze Test

Introduction

  • The Morris water maze test
  • The sample experiment
  • References

Physical Setup

The Morris Water Maze Test in EthoVision XT

  • Arena settings
  • Trial control settings
  • Detection settings
  • Trial list

Acquiring Data

  • Protocol

Data Analysis

  • Data preparation
  • Analysis profiles
  • Swim patterns
  • Gallagher's proximity score

The Novel Object Recognition Test

Introduction

  • The novel object recognition test
  • The sample experiment
  • References

Physical Setup

The NOR Test in EthoVision XT

  • Experiment settings
  • Manual scoring settings
  • Arena settings
  • Trial control settings
  • Detection settings
  • Trial list

Acquiring Data

  • Protocol
  • Score behaviors manually

Data Analysis

  • Data preparation
  • Selecting data
  • Analysis profiles
  • Exploratory behavior
  • Heatmaps

The Elevated Plus Maze Test

Introduction

  • The elevated plus maze test
  • The sample experiment
  • References

Physical Setup

The Elevated Plus Maze Experiment in EthoVision XT

  • Experiment settings
  • Manual scoring settings
  • Arena settings
  • Trial control settings
  • Detection settings
  • Trial list

Acquiring Data

  • Protocol
  • Score behaviors manually

Data Analysis

  • Data preparation
  • Selecting data
  • Visualizing data
  • Analysis profiles

The Sociability Cage Test

Introduction

  • The sociability test (three-chamber paradigm)
  • References

The Sociability Test in EthoVision XT

  • Experiment settings
  • Manual scoring settings
  • Arena settings
  • Detection settings
  • Trial list

Acquiring Data

  • Protocol
  • Score behaviors manually

Data Analysis

  • Data preparation
  • Analysis profiles
  • Visualizing data

The Social Interaction Test

Introduction

  • Measuring social interactions
  • The sample experiment
  • References

Physical Setup

  • Lighting conditions
  • Marked vs. unmarked subjects

The Social Interaction Test in EthoVision XT

  • Create an experiment
  • Experiment settings
  • Manual scoring settings
  • Trial control settings
  • Detection settings
  • Trial list

Carrying Out Your Trials

  • Protocol

Data Analysis

  • Data preparation
  • Analysis profiles
  • Social interaction in detail
  • Statistics and charts
  • Visualizing data

Source: EthoVision XT 18 - Application Manual, Noldus Information Technology

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