Knowledge Base

Nesting Over Latency To Zone

Aim

To pick out the data collected before or after the subject has entered a specific zone.

Example: Calculate the velocity of the subject up to when it entered a zone for the first time.

Nesting vs. Nesting Over Subjects

Notice the difference when there are multiple subjects in the same arena:

• To select data of a subject based on the latency to enter a zone by that subject, choose Latency to zone under Nesting. See the procedure below.
• To select data of a subject based on the latency to enter a zone by other subjects, choose Latency to zone under Nesting over Subjects. There you find the Actors tab where you can specify which subjects (Actors) entered the selected zone. See Nesting Over Subjects.

Procedure

1. In the Data Profile, under Nesting, click the button next to Latency to zone.
2. In the Latency to Zone window, under Settings, next to "Latency to," choose which zone visit you want to use for your data selection.
3. Select the zone of interest and choose from the list at the bottom of the window whether you want to analyze the Interval before zone visit or the Interval after zone visit.
4. Click the Body Points tab (when available), and specify the body points that Latency is based on. Next, select one of the following:
   • All selected points: The data is analyzed before/after all the selected body points were simultaneously in the zone.
   • Any selected point: The data is analyzed before/after at least one of the selected body points entered the zone.
5. Click OK. Proceed with step 4 in How to Select Track Segments.

Notes

• If you select Latency to zone under Nesting, different subjects in the same arena are analyzed in different track segments because the nesting criterion is defined based on each subject separately.
• If you select Latency to zone under Nesting over Subjects, different subjects are analyzed in the same track segments, because the nesting criterion is determined by the Actors specified and is valid for all the subjects. See Nesting Over Subjects.

Nesting Over Head Directed To Zone

Aim

To pick out the data collected when the head of the subject (or more subjects, named Actors) is pointing to a zone or a circular area around a point. The zone or point must be defined in the Arena Settings.

Example: Calculate the velocity of the subject every time it was pointing to the target zone.

Nesting vs. Nesting Over Subjects

Notice the difference when there are multiple subjects in the same arena:

• To select data of a subject based on when that subject points to a zone, choose Head directed to zone under Nesting. See the procedure below.
• To select data of a subject based on when other subjects point to a zone, choose Head directed to zone under Nesting over Subjects. There you find the Actors tab where you can specify which subjects (Actors) were pointing to the selected zone. See Nesting Over Subjects.

Procedure

1. In the Data Profile, under Nesting, click the button next to Head directed to zone.
2. In the Head Directed to Zone window, select:
   • The zone of interest (a zone or a point of interest plus a specific radius).
   • When to calculate the Head directed to zone state. Use this option to restrict the selection criterion (e.g., calculate Head directed to zone only when the subject was in an area around the target zone, not the entire arena). For details about how Head directed to zone is calculated, see Dependent Variables in Detail > Head Directed to Zone.
3. Click OK. Proceed with step 4 in How to Select Track Segments.

See Also

• Nesting Over Subjects

Nesting Over Behavioral States

Aim

To pick out the data collected when the subject was in a specific behavioral state. See the following sections for information on each option:

• Nesting Over Activity State
• Nesting Over Acceleration State
• Nesting Over Body Elongation State
• Nesting Over Body Angle State
• Nesting Over Movement
• Nesting Over Mobility State
• Nesting Over Proximity
• Nesting Over Body Contact
• Nesting Over Relative Movement
• Nesting Over Side by Side
• Nesting Over Behaviors Scored Manually
• Nesting Over Behaviors of Rat/Mouse Behavior Recognition
• Nesting Over Behaviors of Live Mouse Tracker

Important: You may have defined thresholds during acquisition for behavioral states, like Movement, Activity State, or Mobility State in the Analysis Results and Scoring Pane. Those thresholds are not copied to Data Selection. So you have to adjust the thresholds here as well.