Knowledge Base

Opening The DanioVision Casing

Tools You Need

A Phillips or (preferably) a Pozidrive screwdriver, size 2. Do not use electric screwdrivers.

Removing The Bench Plate

Remove the bench plate, for example, to install the Tapping Device and other custom hardware you want to place inside the DanioVision Observation Chamber. It is also helpful to remove the bench plate before opening the top casing.

1. Remove the four screws as indicated in the picture.
2. Remove the bench plate.

Removing The Top Casing

Remove the top casing, for example, when you need to replace the camera.

1. Optionally, remove the bench plate first, as this makes the following steps easier.
2. Remove the eight screws as indicated in the picture. Note that the screws located more internally are shorter than the others. Take note of which screw goes to which hole for when you put everything back in place.
3. Close the lid and lift the top casing.
4. When putting the top casing back in place, make sure that you insert the sides into the bottom casing as indicated in the picture.
5. Tighten all the screws.

Removing The Sides And Back Casing

Remove the sides and back casing to access the connector box, wirings, and internal tubings. In most cases you do not need to do so.

1. Remove the top casing as described in the previous section.
2. Place the DanioVision Observation Chamber just over the edge of the table and remove the three screws from the bottom plate nearest to the side of the chamber. Do not remove the other screws.
3. Repeat the previous step for the other side.
4. Remove the two screws at the back as indicated in the picture.
5. You can now lift the casing.

Removing The Connector Panel

To remove the connector panel at the back of the observation chamber, remove the four screws as indicated in the picture.

Adjusting The Lid Friction

You can adjust the friction of the observation chamber lid. If the lid falls down easily or opens with much friction, loosen or tighten the three screws per hinge, respectively.

Fine Camera Adjustments

Each DanioVision system is thoroughly checked and set to its optimal configuration. However, transport and handling of the system may sometimes cause slight changes in the camera position relative to the well plate, resulting in a sub-optimal video image. This section helps you restore the original settings.

Centering The Well Plate Horizontally

Follow these steps if the well plate is not centered horizontally:

1. Loosen the washer located behind the camera bracket, under the camera profile.
2. Turn the camera in the direction required until the well plate is in the middle of the camera view.
3. Tighten the washer to fix the camera in the new position.

Centering The Well Plate Vertically

If the camera is rotated forward or backward in its vertical plane, the video image may appear off-center vertically.

1. Loosen the two bolts at the sides of the camera profile.
2. Rotate the camera bracket forward or backward until the well plate is centered vertically in the video image.
3. Tighten the bolts.

Correcting A Rotated Well Plate

Follow the steps below if the margin of the well plate is not parallel to the margin of the video image:

1. Open the DanioVision Observation Chamber and locate one of the bolts at the side of the camera profile (either left or right).
2. Loosen the bolt using a 3-mm Allen key.
3. Gently move the camera bracket forward or backward until the well plate is in the correct orientation. When ready, tighten the bolt.

Example Of A Good Video Image

Check that the well plate fills the video image as much as possible, with the letters A–H and numbers 1–8 clearly visible. Set the camera lens to maximum aperture. The image is overexposed when no water is present. Tighten the three screws on the camera lens once the image is optimized.