Knowledge Base

System Specifications

DanioVision DVOC-0050

Dimensions

• 61 x 32 x 46 cm (24 1/64" x 12 19/32" x 18 7/64") (H x W x D) in closed position
• Door opening: 36 x 29 cm (14 11/64" x 11 27/64") (H x W)

Weight

• 15 kg excluding power supply, external tubes, and cables

Power Supply

• Power requirements: 24 Volt DC, 4.0 A (max)
• Mains adapter: input 100–240 Volt AC, output 24 Volt DC, 4.0 A

Camera

• Brand and type: Basler acA1300-60gm
• Interface: Gigabit Ethernet (RJ45 connector, CAT5e or higher)
• Sensor: 1/1.8" Progressive Scan CMOS, monochrome
• Maximum resolution: 1280 x 1024
• Maximum frame rate: 60 fps

Lens

• 12 mm 2 Megapixel, C-mount, F1.4, includes IR pass filter
• Optional lens: 8 mm 2 Megapixel, C-mount, F1.4, includes IR pass filter. Note: this lens requires a 0.5 mm spacer ring.

Video Performance (H x V Pixels)

• Default: 1280 x 960 at 30 fps (in combination with 12 mm lens)
• Optional: 640 x 480 at 60 fps (in combination with 12 mm lens)
• Optional: 800 x 600 at 60 fps (in combination with 8 mm lens)

Maximum frame rates as measured in a 96 well plate setup.

Backlight Unit

• Combined infrared (IR) and white light (White Light Stimulus) array
• Infrared wavelength (typical): 950 nm. According to published research, zebrafish are sensitive to infrared light, though not above 910 nm (see Shcherbakov et al. 2013, PLoS ONE 8(5): e64429). The IR light provided by DanioVision is therefore unlikely to affect the fish's behavior.
• White light color temperature (typical): 5500 ºK
• White light level controllable with EthoVision XT. Intensity range: standard levels 0 to approximately 10,000 lux; low light levels (Low Light switch set to L): 0 to about 20 lux. Light level measured directly at the bottom of the water basin.

Multi Basin

• Water inlet, water outlet, and overflow
• Basin size: 158 x 132 mm (6 7/32" x 5 13/64")
• Supports ANSI/SBS compatible micro plates (L = 127.76 ± 0.5 mm, W = 85.48 ± 0.5 mm) with a maximum height of 27 mm (1 1/16")
• Supports Petri dishes up to 90 mm (3 35/64") in diameter
• Temperature sensor supporting the use of the Noldus DVTCU Temperature Control Unit

Water Connections

• Water inlet/outlet: tube fitting 8 mm
• Water overflow: tube fitting 10 mm

Tubes Included

• 1.5 m clear PVC hose, 8 mm OD, 5 mm ID for water inlet (green color marked)
• 1.5 m clear PVC hose, 8 mm OD, 5 mm ID for water outlet (yellow color marked)
• 1.5 m clear PVC hose, 10 mm OD, 8 mm ID for water overflow (red color marked)

PC Interface

• Ethernet

External I/O

• TTL input/output (2x modular RJ45 connector): 2x 2 TTL input, 2x 2 TTL output
• TTL Trigger Out (2x BNC connector): 2x TTL output

System Start-Up Time (Cold Start)

• Approximately 15 minutes

Cleaning The DanioVision Observation Chamber

• Outside of the chamber: use a proprietary glass-cleaning fluid or a detergent solution.
• Inside parts (Fresnel lens, lens heater, etc.): use only a damp microfiber cloth with a mild detergent solution in warm or lukewarm water. Ensure the cloth is just damp and leaves no droplets on the surface.

DanioVision Temperature Control Unit (TCU)

Dimensions

• 460 x 210 x 280 mm / 18.11" x 8.26" x 11.02" (L x W x H)
• Hose connectors: 8 mm outer diameter tube

Weight

• 7 kg / 15.43 lb

Electrical

• Operating voltage: 24 V DC
• Power consumption (average / maximum): 40 / 120 W
• TTL port specs: 2 input / 2 output, max. 5 V / max. 5 V, open collector

Operational

• Temperature accuracy: ± 0.5 °C / ± 0.9 °F
• Temperature set point range: 15–40 °C / 59–104 °F
• Maximum heating capacity: at least 10 °C / 18 °F above ambient temperature (within the temperature set point range 15–40 °C / 59–104 °F)
• Maximum cooling capacity: 2.5 °C / 4.5 °F below ambient temperature (within the temperature set point range 15–40 °C / 59–104 °F)
• Permissible water temperature range: 5–50 °C / 41–122 °F
• Water capacity: approximately 350 ml / 11.83 oz
• Pump flow rate – water: approximately 310 ml per min / 10.48 oz per min
• Water filter type: 20 μm pore size, 1 inch diameter
• Cooling liquid capacity: approximately 250 ml / 8.45 oz
• Cooling liquid type: colored, anti-corrosive, anti-algae, PC-type cooling liquid
• Pump flow rate – cooling liquid: approximately 1650 ml per min / 55.79 oz per min
• Maximum height difference between DVOC and TCU: 1.3 m / 4.26 ft. Important: do not place the Temperature Control Unit higher than the Observation Chamber.
• Noise level: average over all frequencies less than 35 dB; maximum peak of approximately 48 dB around 160 Hz

Environmental Conditions

• Permissible ambient temperature range: operating 10–35 °C / 50–95 °F; storage 5–65 °C / 41–149 °F
• Permissible relative humidity: 20%–80% (non-condensing)

DanioVision Tapping Device

Dimensions

• Tapping Device (including aluminum bracket): 6 x 24 x 7.5 cm / 2 23/64" x 9 29/64" x 2 61/64" (H x W x D)
• Control box: 2.5 x 10 x 6.5 cm / 63/64" x 3 15/16" x 2 9/16" (H x W x D)

Weight

• Approximately 300 g (Tapping Device, bracket, and control box)
• If not pre-assembled in the DVOC-0050, the package includes mounting material

Power

• Power requirements: 24 V DC, 0.4 A (max)

Features

• Controlled from EthoVision XT 10.1 or later with selectable stimulus intensity (1–8)
• Delivers a discrete tap to the well plate holder
• Maximum tapping rate of approximately 3 taps per second

Connectors

• Molex 2 pin – Power (24 V)
• Molex 4 pin – From Tapping Device to control box
• Multi connector (blue) – For TTL control signal

Compatible DanioVision Chambers

• DVOC-004x, DVOC-0050

DanioVision Toplight Unit

Dimensions

• 270 x 220 x 30 mm / 10 5/8" x 8 21/32" x 1 3/16"

White Light

• Greater than 30,000 lux at 25 cm distance (well plate), 1584 lm
• Color temperature: 4000 °K
• Array of 408 LEDs
• Maximum voltage: 18 V

Red Light

• 192 lm, wavelength 623 nm
• Array of 252 RGB LEDs
• Maximum voltage: 24 V

Green Light

• 384 lm, wavelength 525 nm
• Array of 252 RGB LEDs
• Maximum voltage: 24 V

Blue Light

• 240 lm, wavelength 470 nm
• Array of 252 RGB LEDs
• Maximum voltage: 24 V

Source: DanioVision DVOC-0050 Reference Manual - Appendices, Noldus Information Technology