Knowledge Base

Using the Basin Refill

How much liquid to insert via the syringe depends on the level of evaporation and the duration of the experiment. Add liquid into the basin circuit whenever needed.

During refill, do not switch off the Temperature Control Unit.

1. Open the faucet (On position).
2. Make sure you can see the overflow outlet of the DanioVision Chamber (red marked tube).
3. Gently push the syringe to insert liquid.
4. Carefully watch the overflow tube. Whenever the liquid comes, stop pushing the syringe.

Notes:

• Overflow is a good indication for a 100% filled basin, however it may lag on filling.
• The DVTCU will, whenever it occurs, compensate any temperature fluctuation due to the refill. However, make sure that the liquid temperature in the syringe is not too far from the desired basin temperature.

To Refill the Syringe

1. Make sure the faucet is closed (Off position).
2. Disconnect the syringe.
3. Fill the syringe with basin liquid.
4. Make sure no air is present in the syringe.
5. Connect the syringe back to the faucet tube.

Maintenance and Support

Replace the Filter

The filter inside the DanioVision Temperature Control Unit is a particle filter with a pore size of 20 μm. The filter does not remove bacteria and/or yeast.

The filter can get blocked after you have used the system for a longer period of time. When this happens, you will get an 'insufficient-flow' error message. Please first check the error table. Contact Noldus IT to order a new filter.

Before replacing the filter, drain the system.

1. Make sure to use demineralized or distilled water to prevent small particles (greater than 20 μm) from blocking the filter.
2. Open the lid of the Temperature Control Unit by unscrewing the white plastic screw at the front of the unit. You can use a small screwdriver or a coin to do this.
3. Remove the left side panel by unscrewing the two screws at the top and three screws at the bottom of the side panel.
4. Lift the filter from the clamp and disconnect the tubes at both ends from the filter.
5. Insert the new filter, connect the tubes and push the filter back into the clamp. Make sure the small black arrow on the filter points in the correct direction as indicated in the figure.
6. Put the side panel back in place and close the lid of the Temperature Control Unit.

Replace the Liquid Coolant

1. Open the lid of the Temperature Control Unit by loosening the plastic screw at the front of the unit.
2. Remove the left side panel by unscrewing the screws at the top of the side panel.
3. Locate the cap on top of the reservoir for the liquid coolant. This differs slightly between TCU versions.
4. Unscrew the cap using, for example, a small coin.
5. Use the funnel to fill the reservoir with the liquid coolant. It is recommended to fill it to the maximum level. After refilling the liquid coolant, turn the Operation switch to Fill/Drain to make sure the tubes inside the Temperature Control Unit are also filled with liquid coolant. As a result, the level of coolant in the reservoir might drop a bit. Add more coolant so it reaches the Max level.
6. Screw the cap back on the reservoir.
7. Put the side panel back in place and close the lid of the Temperature Control Unit.

Make sure to regularly check that the level of coolant does not drop below the Min indication on the front of the Temperature Control Unit. If the level is too low, refill the coolant.

Wear and Tear

All Noldus hardware is sold with a two-year guarantee. That means that if it breaks within two years of the delivery date, it will be repaired or replaced at Noldus' expense. Please see the document 'Noldus guarantee' to see what is covered by the guarantee.

Wear and tear caused by regular, normal use of the DanioVision Temperature Control Unit is not covered by the guarantee. Below are some recommendations about environmental conditions and cleaning of the Temperature Control Unit.

Environmental Conditions

See the specifications under Environmental Conditions in the specifications section.

Cleaning the TCU

To clean the DanioVision Temperature Control Unit, use a damp cloth. Do not use chemicals or soap to wash the Temperature Control Unit. Make sure that no liquid gets into the Temperature Control Unit.

Keeping the Tubing Clean

It is very important that the tubes are kept as dry as possible when not using the DanioVision and the TCU. To prevent the growth of algae in the tubing, do the following:

• Always fill the system with demineralized water, and drain it after the end of an experiment.
• Keep all units and the tubes away from sunlight.

Technical Support

If after reading this manual you still have questions, please contact our help desk at my.noldus.com.

Event Data Logger

If you purchased a DanioVision Temperature Control Unit after November 2016, it is equipped with a board and a compact SD card that records performance data during functioning. If you encounter problems when using the Temperature Control Unit, the Noldus Help Desk may ask you to send the files stored in the card. In that case, the Noldus Help Desk will send you instructions for how to do that.