Knowledge Base

What Is DanioVision?

DanioVision™ is a system for high throughput tracking of zebrafish larvae in multi-well plates. It consists of:

The DanioVision Observation Chamber (DVOC-0050)

This chamber contains a transparent holder with backlight that fits an ANSI SBS compatible multi-well microtiter plate, infrared (IR) and white light sources underneath the microtiter plate, and a Gigabit Ethernet (GigE Vision) video camera. The optical design provides a stable and undistorted top view of each well and a controlled and constant environment within the observation chamber.

EthoVision XT

Video-tracking software with the Multiple Arenas Module and Trial & Hardware Control Module. With this module you can adjust the white light stepwise based on time or behavior of your fish. You have the option to connect external devices, such as sound or shock generators.

Note that DanioVision DVOC-0050 works with EthoVision XT 18 and newer, and with an Ethernet connection (RJ45 port) to the EthoVision XT computer.

DanioVision Add-Ons

DanioVision Temperature Control Unit

With the DanioVision Temperature Control Unit you can set up a water flow at constant temperature underneath the well-plate.

DanioVision Tapping Device

With the DanioVision Tapping Device you can trigger a startle response in zebrafish.

DanioVision Toplight Unit

With this add-on you can generate top-down light stimuli in your experiments. Four types of light are available: white, red, blue and green.

DanioVision with Optogenetics

DanioVision can be provided with one to three LEDs that illuminate the well plates based on your Trial Control protocol. The Optogenetics add-on allows you to accurately control and time the application of optogenetic stimulation to up to 96 individuals simultaneously (one for each well in a 96-well plate).

Custom Solutions

As research evolves, so do research needs. If you are interested in a custom solution, don't hesitate to contact us. A few examples of recent custom solutions:

• Light-Dark Grid. Commonly used to investigate anxiety behavior in zebrafish. The grid is placed underneath the well plate (available for 6, 12, 24, 48 or 96 wells) and creates a dark and light half in each well. The grid is IR light translucent, leaving the tracking of the zebrafish unaffected.
• High-Speed Camera. DanioVision is equipped with a camera capable of 1000 frames per second. The aim is to measure the startle response of larvae to a stimulus during a Pre-Pulse Inhibition test (Burgess and Granato 2007).
• Split Basin. The DanioVision basin has been modified so that it receives water at two different temperatures. The aim of this solution is to measure temperature preference in zebrafish larvae.
• Ultraviolet Toplight Unit. This is a modification of the Toplight unit which shines UV light. Light intensity is controlled by EthoVision XT.
• Turbo-Cooling TCU. This is a modified Temperature Control Unit which keeps water at a constant temperature of 12° in a room where temperature is about 25°.

For More Information

Manuals

• The EthoVision XT Help (press F1 in EthoVision XT).
• The EthoVision XT - Trial and Hardware Control - Reference Manual. To open this and other manuals, from the Windows Start menu choose All Apps > Noldus > EthoVision XT 18 Other Documentation.

Sample Experiments

On the EthoVision XT downloads page you can find two sample experiments that make use of DanioVision. Once you have downloaded a sample experiment file (*.evz), you can open it in EthoVision XT via File > Restore Backup.

Experiment Templates

EthoVision XT contains templates that help you set up an experiment with arenas, analysis profiles and other elements already defined.

Support Center

If after reading this manual you still have questions, please contact our Support Center at my.noldus.com.

Source: DanioVision DVOC-0050 Reference Manual - What is DanioVision, Noldus Information Technology