Knowledge Base

The DanioVision Observation Chamber

Important: If you are a user of a DanioVision system with version number DVOC-0020/-0021, you need to upgrade your DanioVision Observation Chamber to be able to connect the Temperature Control Unit. Contact your nearest Noldus office for more information.

Accessories

The DanioVision Temperature Control Unit comes with the following parts:

• A 1.5 m color-coded drain tube.
• A cable for the temperature sensor with a cinch-plug on both ends.
• A 3-m network (UTP) cable to connect the TTL ports on the Temperature Control Unit and the DanioVision Observation Chamber.
• Liquid coolant and a funnel to fill up the coolant.
• The Basin Refill Tool, for filling the DanioVision basin with liquid without opening the DanioVision Observation Chamber.
• A power adapter GS120A24 and a power cord.

Note: For connecting the DanioVision Temperature Control Unit, you also need the color-coded tubes that come with the DanioVision Observation Chamber DVOC-0041.

Connect the DanioVision Temperature Control Unit and the DanioVision Observation Chamber

Basic Connection Scheme

Important: Make sure you place the DanioVision Temperature Control Unit at the same level as the DanioVision Observation Chamber. If that is not possible, place the DanioVision Temperature Control Unit at no more than 1.3 m below the DanioVision Observation Chamber.

Procedure

1. Connect the tube with the green color code to the connectors with the green ring on both the DanioVision Temperature Control Unit and the Observation Chamber. This is the tube through which water flows into the DanioVision Observation Chamber.
2. Connect the tube with the yellow color code to the connectors with the yellow ring on both the DanioVision Temperature Control Unit and the Observation Chamber. This is the tube through which water flows out of the DanioVision Observation Chamber.
3. Connect the tube with the blue color code to the connector with the blue ring on the DanioVision Temperature Control Unit. This is the tube to drain the DanioVision system. Make sure this tube ends in a sink or container.
4. Connect the tube with the red color code to the connector with the red ring on the DanioVision Observation Chamber. This is the tube for the water overflow of the Observation Chamber.

Important: Make sure that the tubes with red and blue color codes end in a sink or container, and are slanted downwards so that water flows spontaneously.

5. Connect the cable with the cinch-plugs to the temperature-sensor connectors on both the DanioVision Temperature Control Unit and Observation Chamber.
6. Optionally, connect the network cable to the TTL port on the DanioVision Temperature Control Unit and TTL port 1 on the Observation Chamber. This allows you to log temperature error messages and potentially use these in trial and hardware control in EthoVision XT.

Important: If you have the DanioVision Observation Chamber version DVOC-0030 or older, you cannot use TTL2, because this port is used by the temperature sensor for the lens heater inside the observation chamber.

7. Connect the DanioVision Temperature Control Unit to the mains socket.
8. Connect the DanioVision Observation Chamber to the EthoVision XT computer. For details, see the Reference Manual - DanioVision Observation Chamber DVOC-0041.

Set Up the DanioVision Temperature Control Unit

Source: DanioVision TCU Reference Manual, Noldus Information Technology