Knowledge Base

Introduction

The combination of the DanioVision Observation Chamber and the Temperature Control Unit allows you to set up a temperature-controlled water flow underneath the well plate in the DanioVision Observation Chamber. The result is a constant temperature in the well-plate throughout an experiment.

If you are a new DanioVision user, you get a new DanioVision Observation Chamber, the Temperature Control Unit and all accessories necessary to work with the DanioVision system.

DanioVision users with a DanioVision Observation Chamber purchased before 25 January 2013 must upgrade their DanioVision Observation Chamber in order to be able to work with the Temperature Control Unit. Please contact your Noldus sales representative for more information.

Workflow

Working with the DanioVision Temperature Control Unit involves the following steps:

1. Set Up Connections: Connect the DanioVision Temperature Control Unit and the Observation Chamber. See page 5.
2. Set Up EthoVision XT: Set up the DanioVision Temperature Control Unit. See page 9.
3. Configure Temperature Alarms (Optional): Configure the temperature alarms in EthoVision XT. See page 15.
4. Carry Out an Experiment: Acquire data with the DanioVision Observation Chamber and the DanioVision Temperature Control Unit. See page 15.

Important: After the experiment, drain the DanioVision Observation Chamber and the DanioVision Temperature Control Unit. Also keep the tubing far from sunlight to prevent algae growth inside the tubing. See Drain the System on page 10.

Other Documentation

• Reference Manual - DanioVision Observation Chamber DVOC-0041: To open this manual, in the Apps screen choose Noldus > EthoVision XT Other Documentation.
• EthoVision XT Help: To open the Help, press F1 in EthoVision XT.

You can download all documentation from www.noldus.com/downloads. Note that you must register and log in to access this page.

Connect the DanioVision Temperature Control Unit and the DanioVision Observation Chamber

Source: DanioVision TCU Reference Manual, Noldus Information Technology