Knowledge Base

Analyze Data - Dependent Variables (Continued)

Social Interaction Parameters

• Side by Side – A parameter indicating when two animals are positioned parallel to each other, reflecting a specific type of social interaction or alignment.
• Train – A parameter used to describe a behavior where one animal follows directly behind another, forming a 'train'.

Live Mouse Tracker

• Approach Rearing Subject
• Drink
• Follow
• Get Away
• Group
• Make and Break Group
• Make Contact
• Move
• Nest
• Nose Contact
• Nose Contact Sequence
• Out of Nest
• Rear
• Social Approach
• Stretch Attend Posture
• Social Escape
• Stop

Trial and Hardware Control

• Trial Control State – A time interval specified by two events of Trial & Hardware Control that occurs during the trial. The interval may occur in two or more instances if the events repeat.
• Trial Control Variable – With this variable you can analyze and visualize the variables defined in the Trial Control Settings.
• Trial Control Event – A point event, with no duration, defined by an element of the Trial & Hardware Control (a condition, action, rule, subrule, or reference).
• Hardware Variables – EthoVision allows you to analyze hardware events in the software. Hardware events can be commands sent to external devices or signals sent back by hardware devices to the USB-IO box.

Multi-Condition Variables

You can create new, customized parameters by combining two or more of the ones mentioned above, including manually or automatically scored events, hardware events, and external data. Other custom-built parameters are available upon request.

JavaScript Variables

You can create custom dependent variables with JavaScript code. With JavaScript you have an almost endless range of possibilities to extract information from your track.

JavaScript variables can be of type continuous, event, or state. You can find JavaScript variables ready to use on the EthoVision 19 Installation package and they are also available on GitHub: https://github.com/noldus/EthoVision-JavaScriptCustomAnalysis

Rat Behavior Recognition

Automatically detected behaviors: Drinking, Eating, Grooming, Jumping, Rearing Supported, Rearing Unsupported, Resting, Sniffing, Walking, Twitching.

• Behavior Probability – The probability of the behavior that is detected. You can use this variable to display behavior probability in the integrated visualization.
• Merged Behavior – You can merge automatically detected behaviors into one group, such as rearing (unsupported and supported) and eating and drinking combined.

All automatically scored behaviors have a duration, except for twitching, which has a frequency.

Mouse Behavior Recognition

Automatically detected behaviors: Drinking, Eating, Grooming, Hopping, Rearing Supported, Rearing Unsupported, Resting, Sniffing, Walking, Digging.

• Behavior Probability – The probability of the behavior that is detected.
• Merged Behavior – You can merge automatically detected behaviors into one group.

Statistics Available

Depending on the parameter, the following statistics are available:

• Frequency
• Latency to first occurrence
• Latency to last occurrence
• Total duration
• Variance
• Standard deviation
• Standard error
• Number
• Mean
• Maximum
• Minimum
• Number of samples used to calculate the statistic

Group statistics available:

• Group mean and standard deviation
• Group mean and standard error
• 5-number summary (Minimum, First Quartile, Median, Third Quartile, Maximum)

For grouped statistics, charts are automatically created. By default, this is a bar chart. When time bins are used, a line chart is created. When specifying a 5-number summary, a box-whiskers chart is created. Charts can also be exported as image files.

Source: EthoVision XT 19 Technical Specifications - Rodent, Noldus Information Technology